What question did this study set out to answer?

The aim is to improve RNA sequencing sensitivity in non-mammalian systems that utilize trans-splicing mechanisms.

June 18, 2026Nature ProtocolsOpen Access

High-resolution single-cell sequencing of trans-spliced mRNA

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The aim is to improve RNA sequencing sensitivity in non-mammalian systems that utilize trans-splicing mechanisms.
Developed a new protocol called SL-Smart-seq3xpress for high-sensitivity single-cell RNA sequencing.
Protocol optimized for organisms like T. brucei that perform trans-splicing.
Field-tested on single-cell sorting and library preparation to complete within 3 days.
Captured over 80% of mRNA molecules in individual T. brucei cells, a significant increase compared to previous techniques.
Enabled high-resolution transcriptomic profiling and inference of genome-level structural variation at the single-cell level.
Successfully applied to analyze transcriptional and genomic heterogeneity in T. brucei and other SL-containing eukaryotes.

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Implication

Randomized trial demonstrates enhanced sensitivity in mRNA detection in non-mammalian cells, suggesting practical applications in transcriptomic studies.

Cite This Study

Cosentino et al. (2026) studied this question.

synapsesocial.com/papers/6a338cc3630953a74978df64 https://doi.org/10.1038/s41596-026-01373-7

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Implication

Randomized trial demonstrates enhanced sensitivity in mRNA detection in non-mammalian cells, suggesting practical applications in transcriptomic studies.

High-resolution single-cell sequencing of trans-spliced mRNA

Key Points

Discussion

Implication

Cite This Study

High-resolution single-cell sequencing of trans-spliced mRNA

Key Points

Discussion

Implication

Cite This Study

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