Treatment with live or heat-killed probiotic Lactobacillus reuteri KUB-AC5 improved cardiac functions and mitigated cardiac mitochondrial dysfunction in obese male rats.
Does probiotic Lactobacillus reuteri KUB-AC5 improve cardiac function and mitochondrial dysfunction in obese rats?
Lactobacillus reuteri KUB-AC5, in both live and heat-killed forms, alleviates obesity-induced cardiac mitochondrial dysfunction and improves cardiac function in a rat model.
Cardiac mitochondrial dysfunction is a crucial mechanism underlying obesity-induced cardiovascular diseases. A close link between obesity and gut microbiota has been revealed, and the benefits of gut microbiota modulation by probiotics have been widely identified. Although the probiotic Lactobacillus reuteri KUB-AC5 exerted anti-inflammatory activity and enhanced the activity of beneficial microbes, the effects of this probiotic on mitochondria in the obese heart have never been investigated. Male Wistar rats were divided into 4 groups to receive either a normal diet (ND; n = 9) or a high-fat and high-calorie diet (HFCD; n = 30) for 24 weeks. At the beginning of week 13, ND-fed rats received vehicle, while HFCD-fed rats were further subdivided into 3 groups (n = 10/group) to receive either vehicle, a live probiotic Lactobacillus reuteri KUB-AC5, or a heat-killed probiotic Lactobacillus reuteri KUB-AC5. At the end of week 24, cardiac functions were evaluated. The rats were then euthanized to enable blood and cardiac ventricle collection. Evidence from the obese rats treated with Lactobacillus reuteri KUB-AC5 in both forms indicated reduced body weight and attenuated insulin resistance. Regarding the heart, obese rats treated with either form of Lactobacillus reuteri KUB-AC5 had improved cardiac functions, mitochondrial dynamics, mitochondrial biogenesis, fat and ketone body utilization, anaplerosis, ATP production, and oxidative phosphorylation. In addition, treatment with this probiotic diminished oxidative stress and restored antioxidative capacity of cardiac mitochondria. Our preclinical findings in male rats highlighted the benefits of the probiotic Lactobacillus reuteri KUB-AC5, given in both live and heat-killed forms, in alleviating obesity-induced cardiac mitochondrial dysfunction.
Thonusin et al. (Wed,) conducted a other in Obesity-induced cardiac mitochondrial dysfunction (n=39). Lactobacillus reuteri KUB-AC5 (live or heat-killed) vs. Vehicle was evaluated on Cardiac functions and mitochondrial dynamics. Treatment with live or heat-killed probiotic Lactobacillus reuteri KUB-AC5 improved cardiac functions and mitigated cardiac mitochondrial dysfunction in obese male rats.