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June 19, 2026Journal of Synchrotron RadiationOpen Access

X-ray fluorescence microscopy exposure estimates using a single excitation energy

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Authors

BRBenjamin RoterNorthwestern UniversityACAndrew M. CrawfordMichigan State UniversityTOThomas V. O’HalloranMichigan State University

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Overview

Calculations estimate elemental exposure in biological tissues, guiding experimental planning for low-concentration metals.

Key Points

  • This research aims to calculate the number of photons needed to detect specific elemental concentrations in biological tissues using single energy excitation.
  • Developed calculations for incident photons per pixel for specified elemental concentrations under far-from-threshold excitation.
  • Calculated radiation dose imparted in a simple model of tissue based on photon exposure.
  • Compared calculations with one specific experimental result for validation.
  • Calculated required photon exposure to detect low-concentration elements in biological tissues.
  • Provided estimates for radiation doses relevant to the imaging process.
  • Identified limitations of approximations used in estimating exposure and dose.

Cite This Study

Roter et al. (2026) studied this question.

synapsesocial.com/papers/6a34dbc465a5b0777af2c668https://doi.org/10.1107/s1600577526004923
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