Experimental infection of wild Mastomys natalensis with the parasitic nematode Trichuris mastomysi

Trichuris muris infections in laboratory mice underpin current understanding of host-parasite interactions, yet the extent to which these insights apply to natural systems remains largely untested. In this study, we experimentally infected wild-caught Mastomys natalensis with Trichuris mastomysi under controlled conditions. Mice were assigned to either a single high-dose infection or repeated low-dose exposure. Larval infection success and intensity were assessed 21 days post-infection. Infection success was high in both exposure groups, and infection dose strongly influenced parasite burden, with high-dose animals carrying significantly more larvae than low-dose animals. These results demonstrate that T. mastomysi readily establishes in wild-caught M. natalensis and that exposure dose primarily affects early larval burden rather than infection success. By experimentally infecting a natural rodent host under controlled conditions, this study links mechanistic laboratory approaches with ecologically relevant host-parasite dynamics in the wild.