Evaluation of cytocompatibility and biodegradation characteristics of three different commercially available resorbable collagen membranes: An in-vitro study

Objectives: This study aimed to evaluate and compare the cytocompatibility and biodegradation characteristics of three commercially available resorbable collagen membranes used in guided tissue regeneration. Methods: An in-vitro study was conducted using L929 mouse fibroblast cells cultured in Dulbecco’s Modified Eagle Medium supplemented with antibiotics and fetal bovine serum. Cytocompatibility was assessed using the alamarBlue® cell viability assay, with fluorescence measured at excitation 530–560 nm and emission 590 nm. Biodegradation was evaluated using the Bradford assay following incubation of membranes in trypsin-EDTA solution, with protein release quantified spectrophotometrically. The membranes tested were Cologide, Healiguide, and Surgicoll. Statistical analysis was performed to determine intergroup differences. Results: All tested membranes demonstrated good cytocompatibility. Surgicoll showed the highest cell viability (97.92%), followed by Healiguide (92.80%) and Cologide (90.69%). Biodegradation analysis revealed that Cologide exhibited the fastest degradation rate, while Surgicoll showed comparatively slower degradation. The differences observed among the groups were statistically significant (p < 0.05). Conclusions: Among the tested membranes, Surgicoll demonstrated superior cytocompatibility, whereas Cologide exhibited faster biodegradation. These variations may be attributed to differences in structural properties such as thickness, porosity, and fiber architecture. The findings highlight the importance of appropriate membrane selection based on clinical requirements in periodontal regenerative procedures.