Antioxidant, anti-inflammatory, FTIR, and GC-MS analysis of the fractions of Tapinanthus bangwensis

Oxidative stress and inflammation contribute substantially to the various chronic diseases in the world. Many routine drugs are available and in use, but a large proportion of them produce undesirable effects. The study investigated the antioxidant and anti-inflammatory properties and sought to identify the likely compounds present in Tapinanthus bangwensis. Antioxidant activity was determined using thiobarbituric acid reactive species (TBARS) and hydrogen peroxide (H202) assays. Bovine Serum Albumin Denaturation (BSAD) and Anti-Proteinase (AP) tests were used to assess anti-inflammatory activity. Identification of the compounds was via gas-chromatography-Mass Spectroscopy (GC-MS), while using FTIR (Fourier transition Infrared) to analyze the functional groups. The TBARs result showed that the reference drug (ascorbic acid), ad HECF 1, exhibited a more substantial inhibition compared to HECF 2. Likewise, the reference drug showed a more substantial inhibitory activity on H202 compared to the fractions. But the inhibition level of HECF 1 increased compared to HECF 2. Aspirin demonstrated a more substantial anti-inflammatory effect compared to the fractions. But that of the HECF 2 was higher compared to the HECF 1. The FTIR result showed the presence of a Carbonyl group (C=O), hydroxyl (OH), and amine group (N-H), suggesting that phenols, carboxylic acids, and fatty acids were likely present. The GC-MS result showed four compounds with reports of antioxidant and anti-inflammatory activity: 2-Pentadecanone, 6, 10, 14-trimethyl, Octacosane, 2, 4-di-tert butyl phenol, and Phytol. Thus, these compounds may contribute to the traditional use of T. bangwensis in treating oxidative and inflammatory diseases.