Engineering a Light-Controllable Fluorescent Protein for Peroxynitrite Detection via Genetic Code Expansion

Gloricelly Roman Arocho; Parthasarathi Das; Sachin Tennakoon; Jared Shaw; Wei Niu; Jiantao Guo

doi:10.1039/d6cb00083e

January 1, 2026RSC Chemical BiologyOpen Access

Engineering a Light-Controllable Fluorescent Protein for Peroxynitrite Detection via Genetic Code Expansion

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Key Points

The study aims to develop a fluorescent protein that can detect peroxynitrite using genetic code expansion techniques.
Developed a photoconvertible fluorescent protein incorporating p-borono-L-phenylalanine.
Engineered the protein from circularly permuted mEos2 for enhanced functionality.
Tested the sensor's ability to respond selectively to peroxynitrite.
Successfully created a fluorescent protein that reacts to peroxynitrite, preserving its photoconversion ability.
Achieved selective detection in various chemical environments, demonstrating its practical application for monitoring peroxynitrite levels.

Implication

Demonstrates selective peroxynitrite detection in live cells, suggesting a novel biosensor approach.

Abstract

We report a noncanonical amino acid-containing photoconvertible fluorescent protein for selective peroxynitrite detection. Incorporation of p-borono-L-phenylalanine into circularly permuted mEos2 affords a peroxynitrite-responsive sensor that retains photoconversion capability, expanding chemical...

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Cite This Study

Arocho et al. (2026) studied this question.

synapsesocial.com/papers/6a362f3bdb0793dc1a536c37 https://doi.org/10.1039/d6cb00083e

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Engineering a Light-Controllable Fluorescent Protein for Peroxynitrite Detection via Genetic Code Expansion

Key Points

Implication

Abstract

Discussion

Cite This Study

Engineering a Light-Controllable Fluorescent Protein for Peroxynitrite Detection via Genetic Code Expansion

Key Points

Implication

Abstract

Discussion

Cite This Study

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