Rapid progressors with type 1 diabetes had significantly higher Caspase-3 levels (adjusted difference +2.12 NPX) and lower von Willebrand Factor compared to complication-free Escapers.
Cross-Sectional (n=149)
Yes
Cardiovascular resilience in long-term Type 1 Diabetes is associated with a distinct proteomic signature compared to rapid progression of vascular complications.
Mean Difference: 2.12 (95% CI 1.61–2.64)
p-value: p=<0.001
Abstract Cardiovascular disease (CVD) is the leading cause of morbidity and mortality in Type 1 Diabetes (T1D), but a subset of individuals remains free from macrovascular or renal complications despite decades of hyperglycaemia and a significant risk factor burden. We used a targeted proteomic approach (Olink Cardiovascular panel III, targeting 92 proteins) to characterize the proteomic profile of cardiovascular resilience in T1D by comparing 92 patients with long-standing T1D (age 59.8 53.2, 69.1, duration 40.0 35.0, 45.2 years) free from macrovascular complications or nephropathy against a reference group of 57 T1D patients with accelerated vascular pathology (age 42.0 32.0, 56.0, duration 22.0 18.0, 27.0 years), proliferative retinopathy and/or nephropathy in relation to diabetes duration, termed Rapid Progressors (RP). Twenty proteins differed significantly between RP and Escapers (False Discovery Rate FDR < 0.05) after adjustment for age, sex, HbA1c, and eGFR: Caspase-3 was significantly higher in RP (Adjusted difference: + 2.12 Normalized Protein eXpression NPX, p < 0.001). Proteins associated with platelet activation and leukocyte adhesion with increased levels in RP included Junctional Adhesion Molecule A (+ 1.40 NPX), Glycoprotein VI (GP6: + 1.29 NPX), and P-Selectin (+ 0.82 NPX) (all p < 0.001). PECAM-1 (+ 0.55 NPX) and TNFRSF14 (+ 0.43 NPX), were also elevated. RP also showed higher levels of metabolic and tissue-remodelling proteins; Transferrin Receptor (+ 0.53 NPX) and Fatty Acid Binding Protein 4 (+ 0.52 NPX), as well as higher Bleomycin Hydrolase, Trefoil Factor 3, GDF-15, U-PAR, and Cystatin B. Conversely, von Willebrand Factor (vWF) levels (− 1.35 NPX, p < 0.001) and Paraoxonase 3 (PON3) was lower in RP (− 0.34 NPX, p = 0.003). In conclusion, escaping complications in long-term T1D appears to be associated with active molecular mechanisms. Progression is marked by apoptosis (Caspase-3), fibrosis (CHI3L1) and platelet activation (GP6), whereas resilience is associated with a distinct signature involving higher vWF and PON3. These findings highlight a profound biological divergence between extreme T1D phenotypes and provide a foundation for further research into vascular resilience.
Ekström et al. (Sat,) conducted a cross-sectional in Type 1 Diabetes (n=149). Rapid Progressor phenotype vs. Escaper phenotype was evaluated on Caspase-3 (CASP-3) normalized protein expression (MD 2.12, 95% CI 1.61 to 2.64, p=<0.001). Rapid progressors with type 1 diabetes had significantly higher Caspase-3 levels (adjusted difference +2.12 NPX) and lower von Willebrand Factor compared to complication-free Escapers.