The progression values of heart rate and biochemical plasma parameters during training of 3-years old warmblood stallions for breeding licensing

Q: What is the clinical evidence from this study?

Study design: Observational. Population: Healthy young sport horses (n=13). Intervention: Standardized free jumping exercise and long-term training vs. Rest. Primary outcome: Heart rate and plasma biochemical parameters (p=< 0.05).

Q: What does this research mean for the field?

Standardized training in young stallions induces distinct acute and long-term physiological and biochemical adaptations, including stable heart rate and lactate levels alongside transient post-exercise shifts in cortisol, NEFA, and proteins. Novelty: ClaimNovelty.INCREMENTAL. Consensus alignment: ConsensusAlignment.NEUTRAL.

Q: What question did this study set out to answer?

The study aims to assess cardiovascular and plasma biochemical responses of training in young sport horses during preparation for breeding licensing.

Hana Dočkalová; Dana Zalesakova; Daria Baholet Bátik; Andrej Bátik; Daniela Kacetlova; Pavel Horký

doi:10.1016/j.jevs.2026.106062

June 1, 2026Journal of Equine Veterinary Science

The progression values of heart rate and biochemical plasma parameters during training of 3-years old warmblood stallions for breeding licensing

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Key result

Acute exercise linked to transient shifts in plasma proteins, cortisol, and NEFA at 15 minutes.

P<0.05
n=13

Why the study?

Physiological adaptation to training in young sport horses remains incompletely characterized, particularly during standardized preparation for breeding licensing.

Does a 112-day training period with standardized free jumping exercise alter heart rate and plasma biochemical parameters in three-year-old Czech Warmblood stallions?

Key Points

The study aims to assess cardiovascular and plasma biochemical responses of training in young sport horses during preparation for breeding licensing.
Monitored 13 Czech Warmblood stallions over a 112-day training period.
Recorded heart rate parameters during standardized free jumping tests.
Assessed plasma biochemical parameters at rest and after exercise at 15 and 60 minutes.
Heart rate parameters and lactate levels remained stable (p > 0.05), indicating appropriate aerobic workload.
Acute exercise led to significant changes in total protein, albumin, cortisol (83.87 ± 3.59 nmol/L), and NEFA (0.13 ± 0.01 mmol/L) 15 minutes post-exercise (p < 0.05).
Long-term adaptations showed reliable trends, including a linear increase in total antioxidant status.

Study Design

Type

Observational (n=13)

Structured PICO

Does a 112-day training period with standardized free jumping exercise alter heart rate and plasma biochemical parameters in three-year-old Czech Warmblood stallions?

P

Population

13 three-year-old Czech Warmblood stallions monitored over a 112-day training period to evaluate physiological adaptations.

E

Exposure

112-day training period with standardized free jumping exercise

O

Outcome

Heart rate parameters (minimum, maximum, and mean) and plasma biochemical parameters at rest and 15 and 60 min after exercisesurrogate

Main Result

p-value: p=< 0.05

Acute exercise and long-term training induce distinct physiological and biochemical adaptations in young stallions, which can be monitored to assess training adequacy.

Implication

Supports monitoring HR and biochemical markers in young stallions; leaves open generalization to broader equine training protocols.

Abstract

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Discussion

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Cite This Study

Dočkalová et al. (2026) conducted an observational in Healthy young sport horses (n=13). Standardized free jumping exercise and long-term training vs. Rest was evaluated on Heart rate and plasma biochemical parameters (p=< 0.05).

synapsesocial.com/papers/6a3723b201fe431c251889d9 https://doi.org/10.1016/j.jevs.2026.106062

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The progression values of heart rate and biochemical plasma parameters during training of 3-years old warmblood stallions for breeding licensing

Key Points

Study Design

Structured PICO

Main Result

Implication

Abstract

Discussion

Cite This Study

The progression values of heart rate and biochemical plasma parameters during training of 3-years old warmblood stallions for breeding licensing

Key Points

Study Design

Structured PICO

Main Result

Implication

Abstract

Discussion

Cite This Study

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