What question did this study set out to answer?

The aim is to explore the binding characteristics of phorbol dibutyrate and epidermal growth factor in human epidermal cells.

Binding of Phorbol Dibutyrate and Epidermal Growth Factor to Cultured Human Epidermal Cells

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Binding of phorbol dibutyrate and epidermal growth factor affects keratinocytes and melanoma cells, suggesting distinct receptor types.

The aim is to explore the binding characteristics of phorbol dibutyrate and epidermal growth factor in human epidermal cells.
Primary cell cultures of normal human epidermal keratinocytes and melanocytes were utilized.
Scatchard analyses were conducted to determine binding site classes and affinity constants.
Receptor binding was assessed using [3H]PDBu and epidermal growth factor in various cell lines.
Keratinocytes exhibited two classes of binding sites for PDBu with a high-affinity constant of 37 nM and a low-affinity constant of 4,880 nM.
Melanoma cultures had lower affinity constants and fewer binding sites compared to keratinocytes.
EGF binding was significant in keratinocytes and primary melanoma cultures but negligible in metastatic melanoma cultures.

Greenebaum et al. (1983) studied this question.

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