Key result
Exposure to Kv1.5 antisense oligodeoxynucleotides caused an approximately 50% reduction in IKur in cultured human atrial myocytes compared to controls, without affecting Ito.
Why the study?
Does antisense oligodeoxynucleotide directed against Kv1.5 mRNA inhibit IKur and Ito in cultured human atrial myocytes?
Population
Cultured adult human atrial myocytes and cultured human ventricular myocytes
Comparison
Antisense phosphorothioate oligodeoxynucleotides… vs Homologous oligodeoxynucleotides with four…
Design
Preclinical
Follow-up
48 hours
Authors
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Supports selective Kv1.5 targeting for IKur modulation in atrial myocytes; leaves open in vivo efficacy and safety.
Does antisense oligodeoxynucleotide directed against Kv1.5 mRNA inhibit IKur and Ito in cultured human atrial myocytes?
Kv1.5 channel subunits are essential to the expression of IKur but do not play a role in Ito in cultured human atrial myocytes.
Feng et al. (1997) studied Cultured adult human atrial and ventricular myocytes. Antisense phosphorothioate oligodeoxynucleotides directed against Kv1.5 mRNA vs. Homologous oligodeoxynucleotides with four mismatch mutations, or vehicle was evaluated on Ultrarapid delayed rectifier (IKur) and transient outward (Ito) currents. Exposure to Kv1.5 antisense oligodeoxynucleotides caused an approximately 50% reduction in IKur in cultured human atrial myocytes compared to controls, without affecting Ito.
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