Synergistic effect of basil, mint extracts and zinc oxide nanoparticles on biochemical parameters in rats reduced by methotrexate

The basil and mint plants were collected from local markets, the plants were extracted using a Soxhlet apparatus with 70% methanol alcohol. Zinc nanoparticles with a typical size of 33–40 nm were acquired from Nanomaterials.Design Eighty adult male rats from the University of Karbala's animal facility were used in this investigation. They were given food. Rat groups were divided into eight groups.G1: Control juste received saline solution (0,90%). G2: 100 mg of Basil and 10 mg of MTX per K.B W. G3: 150 mg of Basil and 10 mg of MTX per K.B W. G4: 100 mg of mint and 10 mg of MTX per K.B W. G5: 150 mg of mint and 10 mg of MTX per K.B W. G6: 0.5 mg of zinc nanoparticles and 10 mg of MTX per K.B W.G7 :0.8 mg of zinc nanoparticles and 10 mg of MTX per K.B W.G8:synergistic effect between basil and mint extracts 150 mg and zinc oxide nanoparticles 0.8mg MTX per K.B W. The combined treatment (basil + mint + nano zinc) achieved the best results compared to the individual treatments, indicating a true synergistic effect that enhances liver protection through the reduction of MDA and LPO levels, an increase in GPX activity, and a relative improvement in total cholesterol and triacylglyceride levels. This suggests the effectiveness of plant antioxidants in reducing lipid peroxidation and enhancing the efficiency of cellular defences.