Proliferative diabetic retinopathy was associated with a 3.72-fold increase in vitreous miR-204-5p and a 2.63-fold increase in miR-126-3p, alongside significantly lower angiopoietin-1 and 2 levels compared to controls.
Case-Control (n=50)
No
Are vitreous levels of angiopoietin-1, angiopoietin-2, Tie-2, and specific microRNAs altered in patients with proliferative diabetic retinopathy compared to non-diabetic controls?
Increased vitreous expression of miR-204-5p and miR-126-3p, along with decreased angiopoietin-1 and 2 levels, may be associated with angiogenesis-related alterations in proliferative diabetic retinopathy.
Effect estimate: Fold change 3.72
p-value: p=0.02
Objectives: This study aims to assess the concentrations of angiopoietin-1, angiopoietin-2, and Tie-2, which are implicated in the pathophysiology of proliferative diabetic retinopathy (PDR), in the vitreous fluid and to evaluate the expression profiles of microRNA (miR)-145-5p, miR-542-3p, miR-5195-3p, miR-126-3p, miR-211-3p, and miR-204-5p. Materials and Methods: The study included 25 patients with PDR and 25 non-diabetic individuals as controls. Vitreous angiopoietin-1, angiopoietin-2, and Tie-2 levels were measured using enzyme-linked immunosorbent assay (ELISA). miRNA expression levels were evaluated using real-time polymerase chain reaction. Results: Vitreous angiopoietin-1 and 2 levels were significantly lower in the PDR group when compared to controls (p0.05). Significantly higher levels of miR-126-3p and miR-204-5p were detected in the PDR group (p0.05). Conclusion: Our data demonstrate that increased miR-204-5p and miR-126-3p expression may be associated with angiogenesis-related alterations in PDR. These findings provide insight into PDR-related angiogenesis and suggest that these microRNAs may represent potential biomarkers of disease-related vascular alterations.
SANCAR et al. (Wed,) conducted a case-control in Proliferative Diabetic Retinopathy (n=50). Proliferative Diabetic Retinopathy vs. Non-diabetic controls was evaluated on Vitreous miR-204-5p expression (Fold change 3.72, p=0.02). Proliferative diabetic retinopathy was associated with a 3.72-fold increase in vitreous miR-204-5p and a 2.63-fold increase in miR-126-3p, alongside significantly lower angiopoietin-1 and 2 levels compared to controls.