A novel A-to-G mutation in circBDP1 alters adipocyte proliferation and differentiation and affects bovine carcass traits

Key Points

• The aim is to investigate how the A-to-G mutation in circBDP1 influences adipocyte proliferation and differentiation and its association with bovine carcass traits.
• Identified circBDP1 gene mutation (rs454813519) using Sanger sequencing and KASP technology.
• Analyzed circBDP1-SNP genotypes in the Shandong Black Cattle Genetic Resource population.
• Conducted EdU assays, flow cytometry, and qRT-PCR on transfected bovine preadipocytes carrying different circBDP1-SNP alleles.
• circBDP1-A allele significantly enhances preadipocyte proliferation and differentiation compared to circBDP1-G.
• The A>G mutation correlates with traits such as muscle size in cows and carcass weight.
• The G allele is hypothesized to originate from Indian zebu cattle.