Elucidating the Optimization Parameters of Essential Oil, Bornyl Acetate and Cisplatin in The Suppression of Cancer Cells Using Response Surface Methodology

The essential oil of Inula graveolens contains a major bioactive compound, bornyl acetate, and is used as a traditional medicine. The chemical agents, such as essential oil, bornyl acetate, and cisplatin, exhibit cytotoxic effects on various cancer cells, including human pulmonary carcinoma (A549), human cervical carcinoma (HeLa), human colorectal carcinoma (HT29), and human breast adenocarcinoma (MCF7), thereby diminishing their viability. Additionally, they have adverse effects on human amniotic cells (normal cells); therefore, this work aims to determine the minimum dose of the agent’s concentration that can maintain the viability of cancer cells at the lowest level without exterminating the human normal cells. Thus, RSM was employed as a mathematical and statistical method for modeling, analyzing, and optimizing agents and their impacts on human normal cells. It has been found that different doses of agents have different effects on cancer cells, and the aim is to optimize the use of agents with fewer trials. Therefore, various agent scenarios have been examined with RSM, and valuable information has been obtained for medical purposes that has been presented in detail.