Key result
Single point mutation of glutamate 3987 to alanine (E3987A) in the mouse RyR2 markedly reduced the sensitivity of the channel to activation by Ca2+.
Population
Mouse cardiac ryanodine receptor (RyR2) expressed in HEK293 cells and studied in planar lipid bilayers
Comparison
Single point mutation of conserved glutamate… vs Wild-type mouse RyR2 channel
Design
Preclinical
Authors
Loading...
E3987A mutation pinpoints a RyR2 Ca2+ sensor in mice; leaves open translation to human arrhythmias or therapies.
Glutamate 3987 is a major determinant of Ca2+ sensitivity for the activation of the mouse cardiac ryanodine receptor (RyR2), providing insights into excitation-contraction coupling.
Li et al. (2001) studied this question. E3987A mutation in mouse RyR2 vs. Wild-type RyR2 channel was evaluated on Sensitivity of the channel to activation by Ca2+. Single point mutation of glutamate 3987 to alanine (E3987A) in the mouse RyR2 markedly reduced the sensitivity of the channel to activation by Ca2+.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: