Scavenging of Reactive Carbonyl Species by Dihydromyricetin and Its Thermal Degradation Products Through Consumption of Hot-Brewed and Cold-Brewed Vine Tea

Reactive carbonyl species (RCS) are highly reactive molecules linked to chronic diseases. Dihydromyricetin (DMY), a high-activity RCS scavenger, exhibited MGO- and ACR-scavenging rates of 70.1% (0.5 h) and 70.3% (2 h) at 37 °C, respectively. Multiple RCS-DMY adducts were characterized using UPLC-MS/MS, and DMY-2MGO-1 and DMY-2ACR-1 were synthesized. RCS adducts formed by DMY and its six degradation products were semiquantified in a DMY-Δ + RCS model system and in human urine and fecal samples after consuming hot-brewed (3 g) and cold-brewed (3 and 6 g) vine tea as DMY carriers. A dose-dependent increase in RCS-adduct excretion was observed in the cold-brewed tea groups, while hot-brewed tea contributed to RCS-scavenging via DMY degradation products (13.5%). No significant difference in RCS-adduct levels was found between the cold-brewed (6 g) and hot-brewed (3 g) tea groups. Overall, these findings suggest that DMY and vine tea are effective RCS scavengers.