Production of antifungal compounds from Bacillus paramycoides LBKURCC218 and Aspergillus fumigatus LBKURCC269 co-cultivation

Bioactive compounds have certain functions in living things, one of which is a compound that acts as an antifungal. Bioactive compounds can be produced by microorganisms, such as bacteria and fungi. In this study, we co-cultured Bacillus paramycoides LBKURCC218 with Aspergillus fumigatus LBKURCC269 to synthesize bioactive compounds. A liquid co-cultivation of these two organisms was tried first. In this study, the antifungal activity and profile of the bioactive compounds produced due to the interaction between bacteria and fungi were investigated. The antifungal activity was determined by the disc diffusion method and the profiles of secondary metabolites from every single culture and co-culture were analyzed by liquid chromatography-mass spectrometer (LC/MS-MS)). The highest antifungal activity test results were obtained from co-cultured fermented ethyl acetate extract of B. paramycoides LBKURCC218 with A. fumigatus LBKURCC269 with a clear zone of 25.67 ± 0.59 mm. The results of LC/MS-MS analysis showed bioactive compounds that are only produced during the co-culture of bacteria and fungi but do not appear in a single culture are rac-Etomidate, 2-Ethyl-2-phenylmalonamide, n-Hexanamide, 7-(tert-butyl)-4-imino-1,2,3,4,5,6-hexahydropyrido[3,4-d]pyridazine-1,5-dione. These results indicate the presence of bioactive compounds induced by co-culture fermentation and their potential as antifungal compounds.