Transplantation of induced pluripotent stem cells-derived cardiomyocytes combined with modified Taohong Siwu decoction improved heart repair after myocardial infarction

Figures

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  Fig. 1. Chemical composition analysis of MTHSWD. (A) The positive ion modes. (B) The negative ion modes.

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  Fig. 2. Identification of iPS and the differentiation of iPS towards cardiomyocytes. (A) The expression of stem cell markers Oct4 and Nanog were detected by immunocytofluorescense staining. (B) Immunofluorescence staining for cTnT in iPS-CMs. Scale bar = 25 μm (B) or 50 μm (C).

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  Fig. 3. Comparison of cardiac function of nude mice in each group after 4 weeks of treatment. (A) The changes in cardiac function were detected by echocardiography. (B–G) The values of EF, FS, LVIDs, LVIDd, LVEDV and LVESV were determined in each group. *P < 0.05 and **P < 0.01, vs the MI group; ##P < 0.01, vs the MTHSWD group; &P < 0.05, vs the iPS-CMs group; MI indicates myocardial infarction; MTHSWD indicates modified Taohong Siwu Decoction. n = 7–8.

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  Fig. 4. Masson's trichrome staining and statistical analysis after 4 weeks of treatment. (A–D) The infarct area and collagen deposition in infarct area of each group was examined by Masson's trichrome staining. (E) The ratio of heart weight to body weight for each group. (F) The ventricular wall thickness of infarct area for each group. **P < 0.01, vs the MI group; ##P < 0.01, vs the MTHSWD group; &P < 0.05 and &&P < 0.01, vs the iPS-CMs group; Scale bar = 50 μm. n = 6.

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  Fig. 5. Effect of MTHSWD on the survival and differentiation of transplanted cells. (A) Double immunofluorescence detection of HNA and cTnT. Scale bar = 25 μm. (B, C) The number of HNA positive cells and percentage of cTnT positive injected cells were analyzed. *P < 0.05, vs the iPS-CMs group. n = 5.

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  Fig. 6. Comparison of endogenous c-kit positive cells in infarct area after 4 weeks of treatment. (A) The expression of c-kit in each group were detected by immunofluorescence staining. (B) The expression of c-kit was analyzed in each group. **P < 0.01, vs the MI group; #P < 0.05, vs the MTHSWD group; &P < 0.05, vs the iPS-CMs group; Scale bar = 25 μm. n = 5.

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  Fig. 7. The expression of cytokines in each group was detected by ELISA. The contents of SDF-1 (A), SCF (B), VEGF (C) and bFGF (D) were detected respectively. *P < 0.05 and **P < 0.01, vs the MI group; #P < 0.05, vs the MTHSWD group; &P < 0.05 and &&P < 0.01, vs the iPS-CMs group. n = 5.