Deposition Plasmonic Gold Nanoparticle Sensors Produced by Pulsed Laser Deposition: Morphological, Structural, and Optical Characterization With Biochemical Sensing via Avidin Binding

Plasmonic gold nanoparticles (AuNPs) have emerged as highly sensitive platforms for biochemical sensing due to their localized surface plasmon resonance (LSPR) properties. In this study, gold nanoparticles were deposited onto glass substrates using pulsed laser deposition (PLD). The morphology of the nanoparticles was characterized using atomic force microscopy (AFM) and SEM, while their crystalline structure was investigated via X-ray diffraction (XRD). LSPR properties were examined using UV-Vis spectroscopy. Furthermore, we explored the potential of the AuNP films as biosensors by observing the shift in the LSPR peak following the binding of avidin molecules. The results show distinct morphological features, crystalline gold phase confirmation, and clear LSPR signatures. Avidin binding induced measurable shifts in the plasmonic peak, confirming the platform's sensitivity to biochemical interactions and refractive index sensing.