Single-molecule studies reveal DnaB helicase's broad fuel adaptability and rapid DNA unwinding in bacteria, implying evolutionary advantages during nucleotide stress.
Key Points
This research investigates how the DnaB helicase unwinds DNA and its efficiency when using various nucleotides as energy sources.
Single-molecule studies on DnaB helicase from Escherichia coli
Assessing translocation speed and nucleotide usage for loading DnaB
Evaluation of DNA duplex displacement under varied salt conditions
DnaB helicase translocates at a speed of up to 3 knt/s, three times faster than the replisome
DnaC can load DnaB using any rNTP or dATP, enabling flexible fuel sourcing
The helicase's translocation supports the use of both ribonucleotides and deoxyribonucleotides.