Determine the Best Conditions for Extracting Bioative Compounds From Rosmarinus Officinalis Leaves

The aim of this study is to study the effect of some factors on the efficiency of ultrasound assisted extraction of some compounds from Rosmarinus officinalis in order to determine the best extraction conditions, detection of Polyphenol compounds in extracts, and test the biological activity of alcoholic and water extracts of leaves of rosemary (Rosmarinus officinalis) against some Gram- negative pathogens (Escherichia. coli) and Gram-positive (Staphylococcus aureus) by agar well diffusion method. The results of extracts showed that the best conditions are: the solvent is ethyl alcohol 85%, temperature is 45 0C, Time is 40 min. alcoholic extract gave a higher extraction yield and Polyphenol content, and more biological activity. the highest biological activity was at concentration (100mg/ml), of alcoholic extract, the average inhibition diameter was 19 mm against Escherichia. coli, and 15 mm against Staphylococcus aureus, while the aqueous extract did not show any activity against the bacteria under study until the concentration 100 mg/ml.