Genome assembly and annotation of the Eastern Mosquitofish (Gambusia holbrooki) sourced from Townsville, Australia.

This record contains the primary annotation and analysis output files for the chromosome-scale genome assembly of an Australian isolate of the Eastern mosquitofish (Gambusia holbrooki) sourced from Townsville, Australia. It accompanies the scripts and documentation repository archived at [https://zenodo.org/records/20659009]. Included files cover the outputs of structural annotation (EGAPx; GTF, CDS, and amino acid sequences), functional annotation (eggNOG-mapper), and repeat annotation (RepeatModeler2/RepeatMasker; repeat families and GTF). The genome assembly itself is available on NCBI under BioProject PRJNA1450840.