Key result
The full-length genomic analysis of three Korean porcine sapelovirus strains revealed a typical picornavirus genome organization with distinct cis-active RNA elements compared to other strains.
The determination of the first complete genome sequences for Korean PSV strains provides foundational data for future investigations into the molecular pathogenesis and evolution of the virus.
Establishes Korean PSV reference genomes; leaves open functional and epidemiological studies on strain variations.
Porcine sapelovirus (PSV), a species of the genus Sapelovirus within the family Picornaviridae, is associated with diarrhea, pneumonia, severe neurological disorders, and reproductive failure in pigs. However, the structural features of the complete PSV genome remain largely unknown. To analyze the structural features of PSV genomes, the full-length nucleotide sequences of three Korean PSV strains were determined and analyzed using bioinformatic techniques in comparison with other known PSV strains. The Korean PSV genomes ranged from 7,542 to 7,566 nucleotides excluding the 3' poly(A) tail, and showed the typical picornavirus genome organization; 5'untranslated region (UTR)-L-VP4-VP2-VP3-VP1-2A-2B-2C-3A-3B-3C-3D-3'UTR. Three distinct cis-active RNA elements, the internal ribosome entry site (IRES) in the 5'UTR, a cis-replication element (CRE) in the 2C coding region and 3'UTR were identified and their structures were predicted. Interestingly, the structural features of the CRE and 3'UTR were different between PSV strains. The availability of these first complete genome sequences for PSV strains will facilitate future investigations of the molecular pathogenesis and evolutionary characteristics of PSV.
No takes yet. Share an insight, caveat, or question.
Son et al. (2014) studied Porcine sapelovirus infection (n=3). Genomic sequencing and analysis vs. Other known porcine sapelovirus strains was evaluated on Full-length nucleotide sequence and structural features. The full-length genomic analysis of three Korean porcine sapelovirus strains revealed a typical picornavirus genome organization with distinct cis-active RNA elements compared to other strains.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: