Protective Effects of Polydatin in Methotrexate-Induced Liver Toxicity in Rats; Multi-Pathway Analysis

Q: What are the key findings of this study?

Polydatin dose-dependently reversed methotrexate-induced hepatotoxicity in rats, with 100 mg/kg normalizing AST/ALT and MDA levels more effectively than 50 mg/kg.

Q: What is the clinical evidence from this study?

Population: Methotrexate (MTX)-induced hepatotoxicity (n=35). Intervention: Polydatin vs. MTX-only (20 mg/kg) and Control. Primary outcome: Serum AST/ALT levels, hepatic malondialdehyde (MDA), and pro-inflammatory/apoptotic markers.

Q: What does this research mean for the field?

Polydatin dose-dependently protects against methotrexate-induced hepatotoxicity in rats by reducing oxidative stress, inflammation, and apoptosis, and restoring tissue-repair signals. Novelty: ClaimNovelty.INCREMENTAL. Consensus alignment: ConsensusAlignment.NEUTRAL.

Selman Gencer; Özge Kandemir; Nurhan Akaras; Hasan Şimşek

doi:10.46810/tdfd.1771398

July 1, 2026Türk doğa ve fen dergisi :/Türk doğa ve fen dergisiOpen Access

Protective Effects of Polydatin in Methotrexate-Induced Liver Toxicity in Rats; Multi-Pathway Analysis

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Key result

Polydatin dose-dependently reverses methotrexate-induced hepatotoxicity in rats, normalizing AST/ALT best at 100 mg/kg.

n=35

Why the study?

This study evaluated polydatin's protective effects against methotrexate-induced hepatotoxicity in rats.

Does polydatin prevent methotrexate-induced hepatotoxicity in Wistar albino rats?

Key Points

This study aims to assess the protective effects of polydatin on liver toxicity caused by methotrexate in rats.
Thirty-five Wistar albino rats were divided into five groups: Control, Polydatin-only, MTX-only, MTX + Polydatin (50 mg/kg), and MTX + Polydatin (100 mg/kg).
Treatments lasted for 7 days.
Biochemical markers including AST, ALT, and inflammatory apoptotic markers were assessed post-treatment.
MTX significantly increased serum AST/ALT levels and hepatic malondialdehyde (MDA) concentrations.
Co-treatment with polydatin at 100 mg/kg dose-dependently normalized AST/ALT and MDA levels, showing superior effects compared to the 50 mg/kg dose (p<0.05).
MTX elevated pro-inflammatory markers (NF-κB, TNF-α, Bax, Caspase-3) while decreasing anti-apoptotic Bcl-2, all of which were reversed by polydatin.

Structured PICO

Does polydatin prevent methotrexate-induced hepatotoxicity in Wistar albino rats?

P

Population

35 Wistar albino rats treated for 7 days to evaluate polydatin's protective effects against methotrexate-induced hepatotoxicity.

I

Intervention

Polydatin (50 mg/kg or 100 mg/kg) co-administered with methotrexate (20 mg/kg) for 7 days

C

Comparator

Methotrexate-only (20 mg/kg), Polydatin-only (100 mg/kg), and untreated Control

O

Outcome

Serum AST/ALT levels, hepatic malondialdehyde (MDA), and pro-inflammatory/apoptotic markers (NF-κB, TNF-α, Bax, Caspase-3 mRNA, Bcl-2, Wnt3 mRNA)surrogate

Polydatin demonstrates dose-dependent hepatoprotective effects against methotrexate-induced liver toxicity in a rat model by modulating inflammatory and apoptotic pathways.

Implication

Hypothesis-generating for polydatin in methotrexate hepatotoxicity; human trials needed before clinical consideration.

Abstract

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Discussion

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Cite This Study

Gencer et al. (2026) studied Methotrexate (MTX)-induced hepatotoxicity (n=35). Polydatin vs. MTX-only (20 mg/kg) and Control was evaluated on Serum AST/ALT levels, hepatic malondialdehyde (MDA), and pro-inflammatory/apoptotic markers. Polydatin dose-dependently reversed methotrexate-induced hepatotoxicity in rats, with 100 mg/kg normalizing AST/ALT and MDA levels more effectively than 50 mg/kg.

synapsesocial.com/papers/6a47520e5c29257aa2578f0c https://doi.org/10.46810/tdfd.1771398

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Protective Effects of Polydatin in Methotrexate-Induced Liver Toxicity in Rats; Multi-Pathway Analysis

Key Points

Structured PICO

Implication

Abstract

Discussion

Cite This Study

Protective Effects of Polydatin in Methotrexate-Induced Liver Toxicity in Rats; Multi-Pathway Analysis

Key Points

Structured PICO

Implication

Abstract

Discussion

Cite This Study

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