Key result
Gallic acid reduced methotrexate-induced elevation of AST, ALT, and ALP, and improved oxidative stress parameters and histological changes in the liver of rats.
Why the study?
Does gallic acid prevent methotrexate-induced oxidative stress and liver damage in rats?
RCT (n=28)
randomly divided
Does gallic acid prevent methotrexate-induced oxidative stress and liver damage in rats?
Gallic acid ameliorates biochemical and oxidative stress parameters in the liver of rats exposed to methotrexate.
Hypothesis-generating for gallic acid in MTX hepatoprotection; should not change clinical practice.
BACKGROUND: Methotrexate, as a chemotherapy drug, can cause chronic liver damage and oxidative stress. Aim of this study was to evaluate the preventive effect of gallic acid (GA) on methotrexate (MTX)-induced oxidative stress in rat liver. METHODS: Twenty-eight male rats were randomly divided into four groups as control, MTX (20 mg/kg, i.p.), MTX + GA (30 mg/kg/day, orally) and GA treated. Aspartate aminotransferase (AST), alanine aminotransferase (ALT) and alkaline phosphatase (ALP) were used as biochemical markers of MTX-induced hepatic injury. Malondialdehyde (MDA) and glutathione (GSH) levels and hepatic antioxidant enzymes activities including catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GPx) were assayed in liver tissue. The expression of SOD2 and GPx1 genes were evaluated by real-time RT-PCR and liver histopathology was evaluated by light microscopy. RESULTS: The result obtained from current study showed that GA remarkably reduced MTX-induced elevation of AST, ALT and ALP and increased MTX-induced reduction in GSH content, GPx, CAT and SOD activity as well as GPx1 and SOD2 gene expressions. Histological results showed that MTX led to liver damage and GA could improve histological changes. CONCLUSIONS: Our results indicate that GA ameliorates biochemical and oxidative stress parameters in the liver of rats exposed to MTX.
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Safaei et al. (2017) conducted an RCT in methotrexate-induced toxicity (n=28). gallic acid vs. methotrexate alone, control, and gallic acid alone was evaluated on biochemical markers of hepatic injury, oxidative stress parameters, and liver histopathology. Gallic acid reduced methotrexate-induced elevation of AST, ALT, and ALP, and improved oxidative stress parameters and histological changes in the liver of rats.
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