Integrated proteogenomic analysis identified severe and mild molecular subtypes of hypertrophic cardiomyopathy, with the severe subtype characterized by reduced fatty acid metabolism and poorer prognosis.
Observational (n=132)
Proteogenomic profiling of hypertrophic cardiomyopathy reveals a severe subtype driven by reduced fatty acid metabolism, which can be targeted therapeutically with baicalin.
BACKGROUND: Hypertrophic cardiomyopathy (HCM) is a heterogeneous disease with diverse prognosis. The underlying mechanisms remain unknown, resulting in limited risk stratification and therapeutic strategies. This study aimed to elucidate molecular subtypes of HCM through integrated proteogenomic analysis and explore subtype-specific therapeutic strategies. METHODS: We conducted an integrated proteogenomic analysis of 132 patients with HCM using myocardial samples, incorporating whole-exome sequencing, RNA sequencing, and proteomics. Unsupervised clustering was used to identify HCM subtypes, which were validated in heart tissues and human induced pluripotent stem cell-derived cardiomyocytes from 2 independent HCM subsets. Subtype-specific signatures and pathways were explored, and their causal link with HCM pathogenesis was established by genetic evidence. A subtype-specific drug was screened using in silico drug prediction, followed by in vitro and in vivo therapeutic effect assessments. RESULTS: Integrated multi-omics analysis identified 2 proteome-based molecular subtypes, severe and mild. We identified 550 subtype-signature proteins, and enrichment analysis based on which revealed that the reduction in fatty acid metabolism and oxidative phosphorylation pathways in HCM versus healthy controls was predominantly driven by the severe subtype, with more severe clinical characteristics and poorer prognosis compared with the mild subtype. Validation in independent cohorts confirmed the robustness of the proteomic subtypes and their association with clinical severity. Additionally, key proteins in fatty acid oxidation and oxidative phosphorylation exhibited consistent expression differences among healthy controls and 2 HCM subtypes. Furthermore, genetic evidence established a causal link between reduced fatty acid oxidation and HCM pathogenesis. Baicalin, identified as a fatty acid oxidation facilitator, improved metabolic and hypertrophic phenotypes in severe subtype human induced pluripotent stem cell-derived cardiomyocytes and Myh6 R404Q/+ mice. CONCLUSIONS: Our analysis demonstrates the metabolic heterogeneity of HCM and enables the development of risk stratification and subtype-specific therapeutic strategies. REGISTRATION: URL: https://www.clinicaltrials.gov ; Unique identifier: NCT03076580.
Ma et al. (Wed,) conducted a observational in Hypertrophic cardiomyopathy (HCM) (n=132). Severe proteomic subtype vs. Mild subtype and healthy controls was evaluated on Molecular subtypes and subtype-specific pathways. Integrated proteogenomic analysis identified severe and mild molecular subtypes of hypertrophic cardiomyopathy, with the severe subtype characterized by reduced fatty acid metabolism and poorer prognosis.