What is the clinical evidence from this study?

Study design: Other. Population: Enterovirus infection. Intervention: 5' UTR recombination vs. Parental MAD4 strain. Primary outcome: Viral fitness and neurovirulence.

November 12, 2015PLoS PathogensOpen Access

Evolution and Emergence of Enteroviruses through Intra- and Inter-species Recombination: Plasticity and Phenotypic Impact of Modular Genetic Exchanges in the 5’ Untranslated Region

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Key result

Recombination of the cVDPV genome with the 5' UTRs of group I enteroviruses (EV-C and -D) was evolutionarily neutral or associated with a gain in fitness, whereas recombination with group II decreased viral replication and virulence.

Population

Experimental model involving cells co-transfected with a bipartite PV/CV-A cVDPV genome rendered defective…

Design

Preclinical

Authors

CMClaire MuslinUniversidad de Las Américas MJMarie‐Line JoffretCentre National de la Recherche Scientifique IPIsabelle PelletierCégep Marie-Victorin

Discussion

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Implication

Recombination may drive enterovirus emergence; leaves open relevance to human cVDPV outbreaks.

Structured PICO

Population

Experimental model involving cells co-transfected with a bipartite PV/CV-A cVDPV genome rendered defective by mutations in the cloverleaf (CL) and 5' UTR RNAs from four human enterovirus species (EV-A to -D)

Intervention

Co-transfection with 5' UTR RNAs from human EV species (EV-A to -D)

Outcome

Rescue of defective cVDPV by recombination, viral replication, and virulence

This study demonstrates the striking plasticity of the enterovirus 5' UTR and how intra- or inter-species modular genetic exchanges can drive the evolution and emergence of new recombinant viral lineages.

Limitations

The specific restriction of the class B recombinants in L20B cells remains to be elucidated.
In vivo and in vitro experiments involved only a limited number of selected recombinants.

Cite This Study

Muslin et al. (2015) studied Enterovirus infection. 5' UTR recombination vs. Parental MAD4 strain was evaluated on Viral fitness and neurovirulence. Recombination of the cVDPV genome with the 5' UTRs of group I enteroviruses (EV-C and -D) was evolutionarily neutral or associated with a gain in fitness, whereas recombination with group II decreased viral replication and virulence.

synapsesocial.com/papers/6a499f99baf9dfe185df03b4 https://doi.org/10.1371/journal.ppat.1005266

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Evolution and Emergence of Enteroviruses through Intra- and Inter-species Recombination: Plasticity and Phenotypic Impact of Modular Genetic Exchanges in the 5’ Untranslated Region

Discussion

Member takes

Implication

Structured PICO

Limitations

Cite This Study

Evolution and Emergence of Enteroviruses through Intra- and Inter-species Recombination: Plasticity and Phenotypic Impact of Modular Genetic Exchanges in the 5’ Untranslated Region

Discussion

Member takes

Implication

Structured PICO

Limitations

Cite This Study

Also Consider

Also Consider