What does this research mean for the field?

Targeted modifications of SARS-CoV-2-neutralizing nanobodies through multimerization and conjugation to antiviral or cytotoxic payloads significantly enhance their binding affinity and neutralizing efficacy against viral variants in vitro. Novelty: ClaimNovelty.INCREMENTAL. Consensus alignment: ConsensusAlignment.NEUTRAL.

What question did this study set out to answer?

The study aims to improve the antiviral activity of SARS-CoV-2 neutralizing nanobodies via multimerization and conjugation with cytotoxic payloads.

July 5, 2026ACS OmegaOpen Access

Multimerization and Conjugation to Antiviral and Cytotoxic Payloads Enhance the Activity of Virus–Neutralizing Nanobodies In Vitro

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Authors

VZV. A. ZhuchkovKazan Federal University NKNatalia A. KruglovaGene Therapy Laboratory PKPolina N. KamzeevaInstitute of Bioorganic Chemistry

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Implication

Randomized trial investigates enhanced antiviral efficacy of engineered nanobodies, suggesting a novel therapeutic approach.

Key Points

The study aims to improve the antiviral activity of SARS-CoV-2 neutralizing nanobodies via multimerization and conjugation with cytotoxic payloads.
Engineered three high-affinity nanobodies (1H3, 2F8, 1D10) against SARS-CoV-2 through oligomerization and conjugation.
Evaluated the neutralizing activity against the Omicron variant (B.1.1.529) and measured enhancements in affinity and potency.
Used Sortase A-mediated conjugation for linking nanobodies to antiviral and cytotoxic payloads.
Increased molecular weight and PEG-mediated tetramerization resulted in higher affinity nanobodies.
Fc fusion significantly improved neutralizing potency against the Omicron variant.
Conjugation with ARVI11 enhanced antiviral efficacy, dependent on the linker type used.

Cite This Study

Zhuchkov et al. (2026) studied this question.

synapsesocial.com/papers/6a49f411f5d1d45b287ffc84 https://doi.org/10.1021/acsomega.6c01932

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Multimerization and Conjugation to Antiviral and Cytotoxic Payloads Enhance the Activity of Virus–Neutralizing Nanobodies In Vitro

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Key Points

Cite This Study

Multimerization and Conjugation to Antiviral and Cytotoxic Payloads Enhance the Activity of Virus–Neutralizing Nanobodies In Vitro

Discussion

Member takes

Implication

Key Points

Cite This Study

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