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July 6, 2026Journal of Liquid Chromatography &amp Related Technologies

Separation and purification of melittin from bee venom using high-speed countercurrent chromatography and pH-zone refining countercurrent chromatography

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Authors

MYMuyun YangTHTian HanLWLanjun Wu

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Overview

Randomized trial develops and compares two methods for isolating melittin in crude bee venom, highlighting efficiency.

Key Points

  • This research focuses on developing and contrasting two chromatographic methods for purifying melittin from bee venom.
  • Utilized high-speed countercurrent chromatography (HSCCC) with a ternary solvent system.
  • Employed pH-zone refining countercurrent chromatography (PZRCCC) with a quaternary solvent system.
  • Confirmed purity and bioactivity using UPLC–MS, peptide sequencing, and antibacterial assays.
  • HSCCC achieved melittin purity exceeding 96.6%, while PZRCCC yielded a purity of 95.2%.
  • Both methods demonstrated effective recovery yields for melittin from crude bee venom.
  • Purified melittin exhibited confirmed identity and bioactivity through various analytical techniques.

Cite This Study

Yang et al. (2026) studied this question.

synapsesocial.com/papers/6a4b453d997070ff83b5b06dhttps://doi.org/10.1080/10826076.2026.2685482
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