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In this study, biosynthesis of self-assembled gold nanoparticles (GNPs) was accomplished using an aqueous extract of green microalga, Chlorella vulgaris . The optical, physical, chemical and bactericidal properties of the GNPs were investigated to identify their average shape and size, crystal nature, surface chemistry and toxicity, via UV–visible spectroscopy, scanning electron microscopy, transmission electron microscopy, X-ray diffraction, Fourier transform infrared spectroscopy and antimicrobial activity. The sizes of the spherical self-assembled cores of the synthesized GNPs ranged from 2 to 10 nm. The XRD patterns showed a (111) preferential orientation and the crystalline nature of the GNPs. The results of the FTIR analysis suggested that the peptides, proteins, phenol and flavonoid carried out the dual function of effective Au III reduction and successful capping of the GNPs. Human pathogen Candida albicans and Staphylococcus aureus were susceptible to synthesized aqueous GNPs. Thus, biosynthesis, stabilization and self-assembly of the GNPs by Chlorella vulgaris extract can be an example of green chemistry and effective drug in the medicinal field.
Annamalai et al. (Thu,) studied this question.