Higher-Order Clustering of Receptors Real-Time Projected by Plasmon-ruler on the Single Live Cell

modulation of heterotypic MET and TfR higher-order clustering at the single-cell level. Under dark-field microscopy (DFM), controllable GNP assembly projects continuous spatiotemporal receptor transitions from loose to dense states, with plasmon scattering converted into pseudocolors for direct visualization. Biological assays confirmed a strong correlation between color changes and the degree of clustering, reducing Met and Akt phosphorylation by ∼2.3- and ∼1.5-fold, and decreasing cell migration from 71.6% to 50.1%. This plasmon-ruler establishes a new strategy for multidimensional imaging of receptor clustering dynamics.