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July 8, 2026RuminantsOpen Access

Surgical Isolation of a Haemonchus contortus FESC Strain: Morphological and Molecular Characterization for Use in Research

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Authors

CCCésar Cuenca-VerdeRHRosa Isabel Higuera-PiedrahitaHCHéctor Alejandro de la Crúz-Crúz

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Overview

Randomized trial examines the surgical isolation and characterization of a Mexican H. contortus strain in lambs, implying future research utility.

Key Points

  • This study aims to surgically isolate a Mexican strain of Haemonchus contortus and characterize its morphological and molecular features for future research applications.
  • Fecal samples collected from infected sheep to isolate nematodes.
  • Larval cultures performed and infection established in a nematode-free lamb.
  • Molecular characterization conducted using qPCR for taxonomic identification.
  • Three days post-transfer, egg excretion was confirmed in the receptor lamb.
  • A total of 3000, 5000, and 10,000 L3 larvae were used to assess pre-patent period and prolificacy.
  • Molecular techniques successfully identified the strain through sequencing of a 176 bp fragment.

Cite This Study

Cuenca-Verde et al. (2026) studied this question.

synapsesocial.com/papers/6a4dea63d2ea289ef6284229https://doi.org/10.3390/ruminants6030052
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Molecular identification of Haemonchus contortus in sheep from Upper Egypt2024 · 8 citations
  2. 2Multi-omics data elucidate parasite-host-microbiota interactions and resistance to Haemonchus contortus in sheep2024 · 9 citations
  3. 3Analysis of benzimidazole anthelmintic resistance in parasitic nematodes <i>Haemonchus contortus</i> using nested isothermal amplification (PCR)2024 · 1 citations
  4. 4Molecular characterization of Haemonchus contortus in sheep from Palani Hills, Tamil Nadu, India2024
  5. 5Epidemiology and phylogeny of Haemonchus contortus through internal transcribed spacer 2 gene in small ruminants2024 · 10 citations