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July 10, 2026European Journal of Microbiology and ImmunologyOpen Access

Evaluation of a commercial real-time PCR assay targeting Leishmania spp. with microscopically positive skin biopsies obtained from Nigerian patients

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Authors

AJAishatu Ishaq JumareDADanlami Elisha AkafyiAEAlbert Eisenbarth

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Overview

Randomized trial evaluates sensitivity of real-time PCR assay for leishmaniasis, suggesting reliable detection in Nigerian patients.

Key Points

  • This research aims to evaluate the sensitivity of a commercial real-time PCR assay targeting Leishmania spp. in skin biopsies from Nigerian patients diagnosed with cutaneous leishmaniasis.
  • Samples from 50 Nigerian patients were collected and subjected to two types of nucleic acid extraction.
  • Two in-house real-time PCR assays targeting kinetoplast DNA and small subunit rRNA were applied, alongside Sanger sequencing of the hsp70 gene.
  • The VIASURE Leishmania detection kit was utilized for confirming the presence of leishmanial DNA.
  • 30 out of 50 bioptic samples provided interpretable sequences, matching primarily with Leishmania major (60%).
  • The VIASURE assay demonstrated higher sensitivity in detecting leishmanial DNA compared to reference PCR assays.
  • All samples showed the presence of L. major post-extraction.

Cite This Study

Jumare et al. (2026) studied this question.

synapsesocial.com/papers/6a508afd6eeac72a4379ff3ahttps://doi.org/10.1556/1886.2026.00030
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