Cultured Epidermal Autograft Formation With Reduced Platelet-Rich Plasma Concentrations: A Preliminary Report

Cultured epidermal autografts (CEA) reportedly have better cosmesis than traditional autografts and are especially useful in the absence of healthy skin for grafting. To support cell growth and prevent desiccation during cultivation, CEAs require platelet-rich plasma (PRP), which is typically applied daily for 2 weeks. However, repeated withdrawal of blood may be unfeasible in certain patients, and importantly, the optimal PRP concentration for cell growth remains undefined. Identifying lower, yet effective, concentrations may reduce the need for extensive blood collection. In this in vitro observational pilot study, epidermal cells were enzymatically retrieved from full-thickness skin biopsies, exposed to graded PRP dilutions (0%–100%, v/v), and incubated in pediatric incubators at 37°C for 7 days. Histologic assessments were performed on fixed eosin-stained suspensions using light microscopy (4–100×), and macroscopic features (cell density, gel formation, and fluid volume) were evaluated. PRP concentrations of 50% and 75% produced more viscous suspensions with higher cell-to-gel ratios and better histoarchitecture, including keratinocyte encapsulation, epidermal stratification, dermal fiber development, and adnexal structures. Preliminary findings with PRP concentrations ≥50% demonstrated growth patterns comparable to 100%, suggesting that reduced PRP volumes may still support CEA formation. However, further investigation of low-volume protocols that minimize blood withdrawal without compromising therapeutic efficacy is necessary to establish conclusive evidence.