This research aims to characterise the metabolite profile of the stem bark extract of Garcinia microphylla Merr. using Liquid Chromatography-High Resolution Mass Spectrometry (LC-HRMS), evaluate antioxidant and anti-inflammatory properties. LC-HRMS detected the presence of (2 R)-5-hydroxy-7-methoxyflavanone as the most common metabolite. The n-hexane, methylene chloride, ethyl acetate, and ethanol extracts exhibited strong inhibition of 2,2-diphenyl-1-picrylhydrazyl (DPPH•) and 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS•+), heat-induced and hypotonicity-induced red blood cell membrane damage, antiproteinase, and protein denaturation in each solvent. The ethanol extract 20 mg/kgBW showed the highest inhibition of 79.31% compared to standard diclofenac sodium 75.47% at the fifth hour using paw edoema model, and 60 mg/kgBW significantly reduced total leukocytes in exudate fluid (5.33 ± 3.68 × 103/mm3), compared with standard diclofenac sodium (6.46 ± 2.40 × 103/mm3) in carrageenan-induced peritonitis in rats. Garcinia microphylla extract shows promising anti-inflammatory potential influenced by the levels of secondary metabolites present and its antioxidant activity.
Wulandari et al. (Thu,) studied this question.