Key result
Targeted FRET-based biosensors enable real-time visualization of cAMP dynamics within discrete subcellular microdomains in living cardiomyocytes, providing insights into cardiac ion homeostasis.
Why the study?
cAMP acts in discrete subcellular compartments to regulate ion homeostasis and excitation-contraction coupling, necessitating methods to visualize these localized signals in living cardiomyocytes.
This review highlights the utility of targeted biosensors for visualizing cAMP compartmentalization in living cardiomyocytes to better understand cardiac physiology and disease.
Insights from cAMP biosensors remain preclinical; leaves open microdomain-specific targeting as a hypothesis-generating therapeutic avenue.
3',5'-Cyclic adenosine monophosphate (cAMP) is a key second messenger that regulates function of proteins involved in ion homeostasis and cardiac excitation-contraction coupling. Over the last decade, it has been increasingly appreciated that cAMP conveys its numerous effects by acting in discrete subcellular compartments or "microdomains." In this mini review, we describe how such localized signals can be visualized in living cardiomyocytes to better understand cardiac physiology and disease. Special focus is made on targeted biosensors that can be used to resolve second messenger signals within nanometers of cardiac ion channels and transporters. Potential directions for future research and the translational importance of cAMP compartmentalization are discussed.
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Dikolayev et al. (2019) conducted a review in Cardiac physiology and disease (cAMP microdomains). FRET-based biosensors for cAMP imaging was evaluated. Targeted FRET-based biosensors enable real-time visualization of cAMP dynamics within discrete subcellular microdomains in living cardiomyocytes, providing insights into cardiac ion homeostasis.
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