= 5.39 ± 0.30 μM) and acted as a mixed-type inhibitor (Ki = 0.29, Kis = 21.3), with stronger affinity for the free enzyme. Its binding mode and conformational stability were further elucidated by molecular docking and molecular dynamics simulations. Among the known isolates, quercetin (8) and afzelin (12) showed moderate inhibitory activities against both α-glucosidase and pancreatic lipase. This work enriches the chemical and pharmacological knowledge of B. brachycarpa and underscores its potential as a source of multitarget enzyme inhibitors.
Yang et al. (Sat,) studied this question.