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Demonstrates improved genome editing in plants, highlighting potential for versatile crop modification.
Native Fanzor2 nucleases exhibit weak editing activity in plants. An optimized Fanzor2 system engineered via ωRNA optimization and structure-based protein mutagenesis achieves robust editing at recalcitrant genomic sites and enables cytosine base editing, supporting versatile crop genome modification via transformation or viral delivery.
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Cao et al. (2026) studied this question.
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