Co-oscillations of vocal membranes and ventricular folds as a novel way of vocalization in rhesus macaques (Macaca mulatta)

These datasets provide supporting materials for the manuscript titled ‘Co-oscillations of vocal membranes and ventricular folds as a novel way of vocalization in rhesus macaques (Macaca mulatta)’. To explore a functional role of ventricular folds in the sound production of rhesus macaques (Macaca mulatta), physical model and excised larynx experiments were carried out. The physical model was constructed based on a micro-computed tomography (micro-CT) measurement of a larynx extracted from a rhesus macaque individual (referred to as the CT model). 1. CT model experimentsExperiments on flow-induced oscillations of the CT model were performed under five settings (VoM, VoF, VeF, VoF-VeF, VoM-VeF). In these settings, two parameters were adjusted. The first one was the ventricular fold position, which determined the distance between the left and right ventricular folds in mediolateral axis to control the adduction level of the ventricular folds. The second one was the vocal fold angle, which controlled the adduction level of the vocal membranes. For each of the 5 settings, 3 model instances were fabricated to be used for the flow experiment. The experiments were repeated three times for each instance. XLSX files contain simultaneous measurements of microphone signal, sound pressure level (SPL), subglottal pressure, and flow-rate (sampling frequency: 12.5 kHz). MP4 files contain high-speed measurements of the dynamics of the CT model (10000 fps). 1.1 VoM.xlsx, VoM_1_1.mp4:The ventricular fold position was set to 3 mm, whereas the vocal fold angle was set to 15 degrees. Vocal membranes (VoM) oscillate solely. XLSX file produces results of Fig. 2d-i. MP4 file produces kymogram of Fig. 2b. 1.2 VoF.xlsx, VoF_1_1.mp4:The ventricular fold position was set to 3 mm, whereas the vocal fold angle was set to 0 degree. Vocal folds (VoF) oscillate solely. XLSX file produces results of Fig. 2d-i. MP4 file produces kymogram of Fig. 2a. 1.3 VeF.xlsx, VeF_1_1.mp4:The ventricular fold position was set to -3 mm, so that only the ventricular folds oscillated. Ventricular folds (VeF) oscillate solely. XLSX file produces results of Fig. 2d-i. MP4 file produces kymogram of Fig. 2c. 1.4 VoF-VeF.xlsx, VoF-VeF_1_1.mp4, VoF-VeF_5_1.mp4:The ventricular fold position was set to -2 mm, whereas the vocal fold angle was set to 0 degree. Vocal folds (VoF) and ventricular folds (VeF) co-oscillate. XLSX file produces results of Fig. 2d-i and Fig. 3a. MP4 files produce kymograms of Fig. 3ce. 1.5 VoM-VeF.xlsx, VoM-VeF_2_1.mp4, VoM-VeF_4_1.mp4:The ventricular fold position was set to -2 mm, whereas the vocal fold angle was set to 15 degree. Vocal membranes (VoM) and ventricular folds (VeF) co-oscillate. XLSX file produces results of Fig. 2d-i and Fig. 3b. MP4 files produce kymograms of Fig. 2df. 2. Excised larynx experimentsExperiments on flow-induced oscillations were performed for four macaque larynges (macaque#1, macaque#2, macaque#3, macaque#4). XLSX files contain simultaneous measurements of microphone signal, sound pressure level (SPL), subglottal pressure, and flow-rate (sampling frequency: 12.5 kHz). MP4 files contain high-speed measurements of the excised larynx experiments (10000 fps). 2.1 macaque#1.xlsx, macaque#1.mp4:Excised larynx experiments of macaque#1. Vocal folds and ventricular folds co-oscillate. XLSX file produces results of Fig. 4a and Fig. 5. MP4 file produces kymogram of Fig. 4c. 2.2 macaque#2.xlsx, macaque#2.mp4:Excised larynx experiments of macaque#2. Vocal folds and ventricular folds co-oscillate. XLSX file produces results of Fig. 5. MP4 file produces kymogram of Fig. 4e. 2.3 macaque#3.xlsx, macaque#3.mp4:Excised larynx experiments of macaque#3. Vocal membranes and ventricular folds co-oscillate.XLSX file produces results of Fig. 5. MP4 file produces kymogram of Fig. 4f. 2.4 macaque#4.xlsx, macaque#4.mp4:Excised larynx experiments of macaque#4. Vocal membranes and ventricular folds co-oscillate. XLSX file produces results of Fig. 4b and Fig. 5. MP4 file produces kymogram of Fig. 4d