Deep Learning–driven synergistic engineering of PET hydrolase for post-consumer PET depolymerization

, representing 2.0- and 1.48-fold improvements over representative high-performance PET hydrolases ICCG and TurboPETase. The depolymerization performance of NI-KYRF towards various post-consumer PET wastes and polyester plastics was significantly improved. At 70 °C, it achieved a 95.5% depolymerization conversion of pc-PET powder within 24 h, and for untreated post-consumer PET film, the conversion reached 80.1%, representing a 2.1-fold improvement in depolymerization efficiency over the parental NI. In addition, enhanced activity toward polyesters plastics, including PBAT and PBT, indicates an expanded substrate scope. Molecular dynamics simulations and structural analysis reveal that these mutations enhance enzyme performance through synergistic mechanisms. Overall, this study establishes a deep learning-guided computational design framework based on EITLEM-Kinetics and demonstrates its effectiveness and broad potential for engineering high-performance PET hydrolases.