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Objective: To compare the cytotoxicity of three dimensional (3D)-printed resins and conventional materials used for orthodontic aligner production. Methods: 3D-printed materials such as 3Dresyn OD-Clear, Clear A, and Tera Harz TC-85 were compared with one thermoplastic material-Duran+. The samples were stored in artificial saliva at 37°C for 7 and 14 days to simulate the oral conditions during one phase of aligner therapy. The saliva was subsequently diluted with cell culture medium to concentrations of 10% (c1), 20% (c2), and 30% (c3). The cytotoxicity of the materials was assessed in human oral fibroblasts using the CCK-8 assay after incubation periods of 4 hours, 24 hours, 48 hours, and 72 hours. Results: > 0.05). Conclusions: Among the tested materials, 3Dresyn OD-Clear and Duran+ demonstrated consistently good biocompatibility, highlighting their potential for safe clinical use. Although the other materials were generally biocompatible, they exhibited cytotoxic effects under certain conditions, underscoring the need for thorough testing to ensure safe clinical use.
Bandić et al. (Fri,) studied this question.