What question did this study set out to answer?

The study investigates how regionalization of the extracellular matrix (ECM) influences cardiac morphogenesis during zebrafish development.

January 8, 2021Cardiovascular ResearchOpen Access

Asymmetric Hapln1a drives regionalized cardiac ECM expansion and promotes heart morphogenesis in zebrafish development

View Full Paper

Ask AI

Bookmark

The study investigates how regionalization of the extracellular matrix (ECM) influences cardiac morphogenesis during zebrafish development.
Live in vivo light sheet imaging of zebrafish embryos was utilized to observe ECM dynamics.
Spatial transcriptomic analysis identified regional gene expression related to ECM expansion.
CRISPR-Cas9 technology was employed to create hapln1a mutants to study its functional role.
Left-sided ECM expansion was observed between the myocardium and endocardium before heart looping, showing regionalized structure.
Hapln1a expression was highest on the future atrium's left side, coinciding with ECM expansion, confirmed by in situ hybridization.
Hapln1a mutants exhibited reduced atrial size and chamber ballooning, showing its critical role in ECM expansion and heart development.

No takes yet. Share an insight, caveat, or question.

Randomized trial identifies ECM expansion as crucial for heart development in zebrafish, highlighting the role of asymmetry cues.

Derrick et al. (2021) studied this question.

View Full Paper

Ask AI

Bookmark