Elucidation of chromatin loops among regulatory regions using Hi-TrAC

Cellular development and differentiation are critically regulated by the spatiotemporal expression of genes within the genome, which are packaged into a highly organized chromatin structure. Genes in the human genome are often regulated by long-distance promoter–enhancer interactions. To better understand gene expression regulation, it is essential to identify specific interactions between distal cis -regulatory elements and their target promoters. To this end, we developed Hi-TrAC, a technique that generates high-resolution interaction maps among accessible chromatin regions without relying on in vitro proximity ligation, while maintaining low sequencing cost. In addition, Hi-TrAC provides a genome-wide landscape of chromatin accessibility. In this article, we present a robust detailed protocol for performing Hi-TrAC assay.