Novel fluorescent biosensor for pathogenic toxins using cyclic polypeptide conjugatesElectronic supplementary information (ESI) available: synthetic approach for microcystin conjugates. See http://www.rsc.org/suppdata/cc/b3/b316057b/

This work describes a two-step conjugate synthesis of a new fluorescent analog of microcystin-LR and its subsequent utilization for the development of an optical biosensor for cyanobacteria toxins. The biosensor concept is based on the competitive binding between the native microcystin and its fluorescent analog at immobilized alkaline phosphatase enzymes.