Illuminating cellular and extracellular vesicle-mediated communication via a split-Nanoluc reporter in vitro and in vivo

Thomas S. van Solinge; Shadi Mahjoum; Stefano Ughetto; Alessandro Sammarco; Marike L. D. Broekman; Xandra O. Breakefield; Killian P. O’Brien

doi:10.1016/j.crmeth.2023.100412

February 1, 2023Cell Reports MethodsOpen Access

Illuminating cellular and extracellular vesicle-mediated communication via a split-Nanoluc reporter in vitro and in vivo

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Key Points

This study aims to evaluate a split Nanoluc reporter system for monitoring functional cellular communication.
Developed a split Nanoluc reporter system for tracking functional transfer between cells.
Performed experiments in vitro and in a subcutaneous tumor model in mice.
Utilized fluorescent labeling to visualize communication pathways.
Demonstrated successful monitoring of direct and indirect communication between cells with a specific focus on extracellular vesicles.
Quantified the functional delivery achieved through the reporter system, enhancing understanding of cellular interactions.

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Implication

Demonstrates functional communication in cellular systems, indicating a novel reporting method's utility.

Abstract

Tools to effectively demonstrate and quantify functional delivery in cellular communication have been lacking. This study reports the use of a fluorescently labeled split Nanoluc reporter system to demonstrate and quantify functional transfer between cells in vitro and in a subcutaneous tumor mouse model. Our construct allows monitoring of direct, indirect, and specifically extracellular vesicle-mediated functional communication.

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Cite This Study

Solinge et al. (2023) studied this question.

synapsesocial.com/papers/6a5d15716368af7686c1faee https://doi.org/10.1016/j.crmeth.2023.100412

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Illuminating cellular and extracellular vesicle-mediated communication via a split-Nanoluc reporter in vitro and in vivo

Key Points

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Implication

Abstract

Discussion

Cite This Study

Illuminating cellular and extracellular vesicle-mediated communication via a split-Nanoluc reporter in vitro and in vivo

Key Points

Ask about this paper

Implication

Abstract

Discussion

Cite This Study

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