Volumetric Properties, Compressibilities and Volume Fluctuations in Phospholipid-Cholesterol Bilayers

We conducted detailed measurements of the apparent specific volume of dipalmitoylphosphatidylcholine (DPPC)–cholesterol mixtures in excess water as a function of pressure up to 70MPa (700bar) at 20, 38 and 50°C. The volumetric properties and the isothermal compressibility κ T of the lipid vesicles were determined at cholesterol concentrations, χ chol , ranging up to 50 mol. The thermodynamic data are compared with other physico-chemical properties of phospholipid–cholesterol mixtures. Furthermore, the thermodynamic properties of the system are discussed in the light of the various T , χ chol –phase diagrams and computer simulation studies published in the literature.