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The stringent response is a conserved stress-adaptation program in bacteria, mediated by the alarmones (p)ppGpp that reprogram transcription, translation, and metabolism. This mini-review surveys mechanistic, physiological, and evolutionary facets of alarmone singnaling across two classes of RelA/SpoT homologue (RSH) enzymes: multidomain-containing long RSHs and small alarmone synthetases/hydrolases (SAS/SAH). We first outline how activation is ribosome-centered: in Escherichia coli, synthesis-only RelA is activated only when bound to a starved ("hungry") ribosome, while SpoT is hydrolase-biased; in Bacillus subtilis, a single bifunctional Rel shifts from hydrolysis- to synthesis-state when locked on a starved ribosome. Beyond (p)ppGpp, SAS enzymes diversify outputs by producing adenosine alarmones such as (p)ppApp and, in toxic SAS (toxSAS) modules, by pyrophosphorylating the tRNA 3'-CCA end. Finally, we discuss evolutionary trajectories-from SAH-SAS operons to fused, ribosome-regulated long RSHs-and argue that pseudo-ZFD motifs in some toxSAS likely represent independent acquisitions.
Kurata et al. (Thu,) studied this question.