Key result
Single-cell RNA sequencing identifies distinct microglial and myeloid subsets in aged post-stroke brains.
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BACKGROUND: Ischemic stroke is a medical emergency that primarily affects the elderly. A complex immune response in the post-stroke brain constitutes a key component of stroke pathophysiology. This study aimed to determine how stroke affects immune cell populations in the aged brain based on molecular profiles of individual cells. METHODS: Single-cell RNA sequencing and a new transient ischemic stroke mouse model with late reperfusion were used. RESULTS: We generated, for the first time, a composite picture of immune cell populations in the stroke aged brain at single-cell resolution. We discovered at least 6 microglial subsets in the stroke aged brain, including a potentially stroke-specific subtype. Moreover, we identified major cell subpopulations formed by infiltrated myeloid cells after stroke, and revealed their unique molecular profiles. CONCLUSIONS: This study provided the first scRNA-seq data set for immune cells in the stroke aged brain, and offered novel insights into post-stroke immune cell heterogeneity.
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Li et al. (2022) studied Ischemic stroke. Transient ischemic stroke with late reperfusion was evaluated on Immune cell populations and molecular profiles at single-cell resolution. Single-cell RNA sequencing of the aged mouse brain after transient ischemic stroke identified at least 6 microglial subsets and major infiltrated myeloid cell subpopulations.
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