Mass spectrometry of Perina nuda picorna-like virus proteins confirmed that the 2A sequence promotes unconventional termination after decoding a glycine codon, supporting the StopGo translation model.
When a eukaryotic mRNA sequence specifying an amino acid motif known as 2A is directly followed by a proline codon, two nonoverlapping proteins are synthesized. From earlier work, the second protein is known to start with this proline codon and is not created by proteolysis. Here we identify the C-terminal amino acid of an upstream 2A-encoded product from Perina nuda picorna-like virus that is glycine specified by the last codon of the 2A-encoding sequence. This is an example of recoding where 2A promotes unconventional termination after decoding of the glycine codon and continued translation beginning with the 3' adjacent proline codon.
Atkins et al. (Tue,) reported a letter. 2A sequence translation (StopGo) was evaluated on Identification of the C-terminal amino acid of the upstream 2A-encoded product. Mass spectrometry of Perina nuda picorna-like virus proteins confirmed that the 2A sequence promotes unconventional termination after decoding a glycine codon, supporting the StopGo translation model.